Fig 1: TMEM106B depletion leads to the accumulation of abnormal NF-containing aggregates in PS19 mice(A, B) Representative images of NF-L, hTau, and β-III-tubulin co-immunostainings in CA3 region in the hippocampus of 8.5-month-old WT, Tmem106b−/−, PS19, and Tmem106b−/− PS19 mice brain sections. The relative fluorescent intensity and number of fluorescent puncta of NF-L were quantified in B. n=6. Data presented as mean ± SEM. Unpaired Student’s t-test: **, p<0.01. Scale bar: 20 μm.(C, D) Western blot analysis of NF-L, p-NF-H/M, TMEM106B, and GAPDH in RIPA-soluble fraction from brain lysates of 6-month-old WT and Tmem106b−/− mice. Relative levels of indicated proteins were quantified in D. n=7. Data presented as mean ± SEM. Unpaired Student’s t-test: **, p<0.01.